The aims of this study were (a) to investigate the acute effects of different drop jump (DJ) accentuated eccentric loading (AEL) protocols and (b) to investigate the effect of these AEL DJ protocols on subsequent countermovement jump (CMJ) performance. The subjects were 12 strength-trained athletes; initially, baseline CMJ performance was assessed and individual optimal DJ drop height identified. In subsequent weeks, subjects completed 1 set of 5 DJs with no additional load or an AEL of 10, 20, or 30% of their individual body mass (BM) using dumbbells to provide the extra load. After the AEL DJ protocols, 3 CMJs were completed after 2, 6, and 12 minutes rest. A generalized linear mixed model was used to investigate the effects of AEL load and time post-DJs on CMJ height, peak power, and ground reaction force. The 20% AEL condition resulted in greater CMJ height in comparison with all other conditions (p < 0.001). CMJ height was significantly greater after 2 and 6 minutes rest compared with 12 minutes (p < 0.001 and p ≤ 0.05 respectively). Greater peak power was also found during the CMJs after the 20% AEL condition compared with baseline, BM, and 10% AEL (p ≤ 0.05). In conclusion, 5 DJs with 20% AEL followed by a 2 minutes recovery period resulted in significant enhancement in CMJ height and peak power.