Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress

Mohsen Seifi, Nicole L. Corteen, Johannes J. van der Want, Friedrich Metzger, Jerome D. Swinny

Research output: Contribution to journalArticle

Abstract

The locus coeruleus (LC) nucleus modulates adaptive behavioral responses to stress and dysregulation of LC neuronal activity is implicated in stress-induced mental illnesses. The LC is composed primarily of noradrenergic neurons together with various glial populations. A neuroglia cell-type largely unexplored within the LC is the NG2 cell. NG2 cells serve primarily as oligodendrocyte precursor cells throughout the brain. However, some NG2 cells are in synaptic contact with neurons suggesting a role in information processing. The aim of this study was to neurochemically and anatomically characterize NG2 cells within the rat LC. Furthermore, since NG2 cells have been shown to proliferate in response to traumatic brain injury, we investigated whether such NG2 cells plasticity also occurs in response to emotive insults such as stress. Immunohistochemistry and confocal microscopy revealed that NG2 cells were enriched within the pontine region occupied by the LC. Close inspection revealed that a sub-population of NG2 cells were located within unique indentations of LC noradrenergic somata and were immunoreactive for the neuronal marker NeuN whilst NG2 cell processes formed close appositions with clusters immunoreactive for the inhibitory synaptic marker proteins gephyrin and the GABA-A receptor alpha3-subunit, on noradrenergic dendrites. In addition, LC NG2 cell processes were decorated with vesicular glutamate transporter 2 immunoreactive puncta. Finally, 10 days of repeated restraint stress significantly increased the density of NG2 cells within the LC. The study demonstrates that NG2 IR cells are integral components of the LC cellular network and they exhibit plasticity as a result of emotive challenges. © 2014 Seifi, Corteen, van der Want, Metzger and Swinny.
Original languageEnglish
JournalFrontiers in Neuroanatomy
Volume8
Issue numberMAY
DOIs
Publication statusPublished - 14 May 2014

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Locus Coeruleus
Neuroglia
Vesicular Glutamate Transport Protein 2
Adrenergic Neurons
Oligodendroglia
Carisoprodol
GABA-A Receptors
Cellular Structures
Dendrites
Automatic Data Processing
Confocal Microscopy
Population
Cell Count
Immunohistochemistry
Neurons

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Seifi, Mohsen ; Corteen, Nicole L. ; van der Want, Johannes J. ; Metzger, Friedrich ; Swinny, Jerome D. / Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress. In: Frontiers in Neuroanatomy. 2014 ; Vol. 8, No. MAY.
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abstract = "The locus coeruleus (LC) nucleus modulates adaptive behavioral responses to stress and dysregulation of LC neuronal activity is implicated in stress-induced mental illnesses. The LC is composed primarily of noradrenergic neurons together with various glial populations. A neuroglia cell-type largely unexplored within the LC is the NG2 cell. NG2 cells serve primarily as oligodendrocyte precursor cells throughout the brain. However, some NG2 cells are in synaptic contact with neurons suggesting a role in information processing. The aim of this study was to neurochemically and anatomically characterize NG2 cells within the rat LC. Furthermore, since NG2 cells have been shown to proliferate in response to traumatic brain injury, we investigated whether such NG2 cells plasticity also occurs in response to emotive insults such as stress. Immunohistochemistry and confocal microscopy revealed that NG2 cells were enriched within the pontine region occupied by the LC. Close inspection revealed that a sub-population of NG2 cells were located within unique indentations of LC noradrenergic somata and were immunoreactive for the neuronal marker NeuN whilst NG2 cell processes formed close appositions with clusters immunoreactive for the inhibitory synaptic marker proteins gephyrin and the GABA-A receptor alpha3-subunit, on noradrenergic dendrites. In addition, LC NG2 cell processes were decorated with vesicular glutamate transporter 2 immunoreactive puncta. Finally, 10 days of repeated restraint stress significantly increased the density of NG2 cells within the LC. The study demonstrates that NG2 IR cells are integral components of the LC cellular network and they exhibit plasticity as a result of emotive challenges. {\circledC} 2014 Seifi, Corteen, van der Want, Metzger and Swinny.",
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Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress. / Seifi, Mohsen; Corteen, Nicole L.; van der Want, Johannes J.; Metzger, Friedrich; Swinny, Jerome D.

In: Frontiers in Neuroanatomy, Vol. 8, No. MAY, 14.05.2014.

Research output: Contribution to journalArticle

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T1 - Localization of NG2 immunoreactive neuroglia cells in the rat locus coeruleus and their plasticity in response to stress

AU - Seifi, Mohsen

AU - Corteen, Nicole L.

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AU - Swinny, Jerome D.

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AB - The locus coeruleus (LC) nucleus modulates adaptive behavioral responses to stress and dysregulation of LC neuronal activity is implicated in stress-induced mental illnesses. The LC is composed primarily of noradrenergic neurons together with various glial populations. A neuroglia cell-type largely unexplored within the LC is the NG2 cell. NG2 cells serve primarily as oligodendrocyte precursor cells throughout the brain. However, some NG2 cells are in synaptic contact with neurons suggesting a role in information processing. The aim of this study was to neurochemically and anatomically characterize NG2 cells within the rat LC. Furthermore, since NG2 cells have been shown to proliferate in response to traumatic brain injury, we investigated whether such NG2 cells plasticity also occurs in response to emotive insults such as stress. Immunohistochemistry and confocal microscopy revealed that NG2 cells were enriched within the pontine region occupied by the LC. Close inspection revealed that a sub-population of NG2 cells were located within unique indentations of LC noradrenergic somata and were immunoreactive for the neuronal marker NeuN whilst NG2 cell processes formed close appositions with clusters immunoreactive for the inhibitory synaptic marker proteins gephyrin and the GABA-A receptor alpha3-subunit, on noradrenergic dendrites. In addition, LC NG2 cell processes were decorated with vesicular glutamate transporter 2 immunoreactive puncta. Finally, 10 days of repeated restraint stress significantly increased the density of NG2 cells within the LC. The study demonstrates that NG2 IR cells are integral components of the LC cellular network and they exhibit plasticity as a result of emotive challenges. © 2014 Seifi, Corteen, van der Want, Metzger and Swinny.

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