CYP105-diverse structures, functions and roles in an intriguing family of enzymes in Streptomyces

Suzy C Moody, E Joel Loveridge

Research output: Contribution to journalReview article

Abstract

The cytochromes P450 (CYP or P450) are a large superfamily of haem-containing enzymes found in all domains of life. They catalyse a variety of complex reactions, predominantly mixed-function oxidations, often displaying highly regio- and/or stereospecific chemistry. In streptomycetes, they are predominantly associated with secondary metabolite biosynthetic pathways or with xenobiotic catabolism. Homologues of one family, CYP105, have been found in all Streptomyces species thus far sequenced. This review looks at the diverse biological functions of CYP105s and the biosynthetic/catabolic pathways they are associated with. Examples are presented showing a range of biotransformative abilities and different contexts. As biocatalysts capable of some remarkable chemistry, CYP105s have great biotechnological potential and merit detailed study. Recent developments in biotechnological applications which utilize CYP105s are described, alongside a brief overview of the benefits and drawbacks of using P450s in commercial applications. The role of CYP105s in vivo is in many cases undefined and provides a rich source for further investigation into the functions these enzymes fulfil and the metabolic pathways they participate in, in the natural environment.

Original languageEnglish
Pages (from-to)1549-63
Number of pages15
JournalJournal of Applied Microbiology
Volume117
Issue number6
DOIs
Publication statusPublished - Dec 2014

Fingerprint

Streptomyces
Biosynthetic Pathways
Enzymes
Xenobiotics
Metabolic Networks and Pathways
Heme
Cytochrome P-450 Enzyme System

Cite this

@article{67df0cab1a724340bed2e7f24be510c8,
title = "CYP105-diverse structures, functions and roles in an intriguing family of enzymes in Streptomyces",
abstract = "The cytochromes P450 (CYP or P450) are a large superfamily of haem-containing enzymes found in all domains of life. They catalyse a variety of complex reactions, predominantly mixed-function oxidations, often displaying highly regio- and/or stereospecific chemistry. In streptomycetes, they are predominantly associated with secondary metabolite biosynthetic pathways or with xenobiotic catabolism. Homologues of one family, CYP105, have been found in all Streptomyces species thus far sequenced. This review looks at the diverse biological functions of CYP105s and the biosynthetic/catabolic pathways they are associated with. Examples are presented showing a range of biotransformative abilities and different contexts. As biocatalysts capable of some remarkable chemistry, CYP105s have great biotechnological potential and merit detailed study. Recent developments in biotechnological applications which utilize CYP105s are described, alongside a brief overview of the benefits and drawbacks of using P450s in commercial applications. The role of CYP105s in vivo is in many cases undefined and provides a rich source for further investigation into the functions these enzymes fulfil and the metabolic pathways they participate in, in the natural environment.",
author = "Moody, {Suzy C} and Loveridge, {E Joel}",
note = "{\circledC} 2014 The Authors published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.",
year = "2014",
month = "12",
doi = "10.1111/jam.12662",
language = "English",
volume = "117",
pages = "1549--63",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "6",

}

CYP105-diverse structures, functions and roles in an intriguing family of enzymes in Streptomyces. / Moody, Suzy C; Loveridge, E Joel.

In: Journal of Applied Microbiology, Vol. 117, No. 6, 12.2014, p. 1549-63.

Research output: Contribution to journalReview article

TY - JOUR

T1 - CYP105-diverse structures, functions and roles in an intriguing family of enzymes in Streptomyces

AU - Moody, Suzy C

AU - Loveridge, E Joel

N1 - © 2014 The Authors published by John Wiley & Sons Ltd on behalf of Society for Applied Microbiology.

PY - 2014/12

Y1 - 2014/12

N2 - The cytochromes P450 (CYP or P450) are a large superfamily of haem-containing enzymes found in all domains of life. They catalyse a variety of complex reactions, predominantly mixed-function oxidations, often displaying highly regio- and/or stereospecific chemistry. In streptomycetes, they are predominantly associated with secondary metabolite biosynthetic pathways or with xenobiotic catabolism. Homologues of one family, CYP105, have been found in all Streptomyces species thus far sequenced. This review looks at the diverse biological functions of CYP105s and the biosynthetic/catabolic pathways they are associated with. Examples are presented showing a range of biotransformative abilities and different contexts. As biocatalysts capable of some remarkable chemistry, CYP105s have great biotechnological potential and merit detailed study. Recent developments in biotechnological applications which utilize CYP105s are described, alongside a brief overview of the benefits and drawbacks of using P450s in commercial applications. The role of CYP105s in vivo is in many cases undefined and provides a rich source for further investigation into the functions these enzymes fulfil and the metabolic pathways they participate in, in the natural environment.

AB - The cytochromes P450 (CYP or P450) are a large superfamily of haem-containing enzymes found in all domains of life. They catalyse a variety of complex reactions, predominantly mixed-function oxidations, often displaying highly regio- and/or stereospecific chemistry. In streptomycetes, they are predominantly associated with secondary metabolite biosynthetic pathways or with xenobiotic catabolism. Homologues of one family, CYP105, have been found in all Streptomyces species thus far sequenced. This review looks at the diverse biological functions of CYP105s and the biosynthetic/catabolic pathways they are associated with. Examples are presented showing a range of biotransformative abilities and different contexts. As biocatalysts capable of some remarkable chemistry, CYP105s have great biotechnological potential and merit detailed study. Recent developments in biotechnological applications which utilize CYP105s are described, alongside a brief overview of the benefits and drawbacks of using P450s in commercial applications. The role of CYP105s in vivo is in many cases undefined and provides a rich source for further investigation into the functions these enzymes fulfil and the metabolic pathways they participate in, in the natural environment.

U2 - 10.1111/jam.12662

DO - 10.1111/jam.12662

M3 - Review article

VL - 117

SP - 1549

EP - 1563

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -