Characterization of the mean flow field in the far wake region behind ocean current turbines

Parakram Pyakurel, Wenlong Tian, James H. VanZwieten, Manhar Dhanak

Research output: Chapter in Book/Report/Published conference proceedingChapter

Abstract

Methoxypolyethylene glycol (mPEG) covalently bound to the surface of human red blood cells (hRBCs) has been shown to decrease immunological recognition of hRBC surface antigens (Bradley et al., 2002). However, there is an increasing shortage of hRBC donations, thus making hRBCs scarce and expensive (Davey, 2004; Riess, 2001). The goal of this study is to similarly PEGylate the surface of bovine RBCs (bRBCs) with the aim of reducing the demand on human blood donations needed for blood transfusions. This study investigates the feasibility of modifying the surface of bRBCs with the succinimidyl ester of methoxypolyethylene glycol propionic acid (SPA-mPEG) for use as a potential blood substitute. The oxygen binding affinity of PEGylated bRBCs was moderately increased with increasing initial SPA-mPEG concentrations up to 4 mM when reacted with bRBCs at a hematocrit of 12%. Oxygen transport simulations verified that SPA-mPEG conjugated bRBCs could still transport oxygen to pancreatic islet tissues even under extreme conditions. PEGylated bRBCs reconstituted to a hematocrit of 40% exhibited viscosities on the order of 3 cp, similar to hRBCs at the same hematocrit. Taken together, the results of this study demonstrate the success of PEGylating bRBCs to yield modified cells with oxygen binding, transport and flow properties similar to that of hRBCs
Original languageEnglish
Title of host publicationJournal of Ocean Engineering and Marine Energy
PublisherSpringer International Publishing AG
Pages113-123
Number of pages11
ISBN (Print)0021-8782 (Print)\r0021-8782 (Linking)
DOIs
Publication statusPublished - 1 May 2017

Publication series

NameJournal of Ocean Engineering and Marine Energy
Volume3

Fingerprint

Oceans and Seas
Erythrocytes
Hematocrit
Oxygen
Blood Donors
Blood Substitutes
Feasibility Studies
Surface Antigens
Islets of Langerhans
Viscosity
Blood Transfusion
monomethoxypolyethylene glycol

Cite this

Pyakurel, P., Tian, W., VanZwieten, J. H., & Dhanak, M. (2017). Characterization of the mean flow field in the far wake region behind ocean current turbines. In Journal of Ocean Engineering and Marine Energy (pp. 113-123). (Journal of Ocean Engineering and Marine Energy; Vol. 3). Springer International Publishing AG. https://doi.org/10.1007/s40722-017-0075-9
Pyakurel, Parakram ; Tian, Wenlong ; VanZwieten, James H. ; Dhanak, Manhar. / Characterization of the mean flow field in the far wake region behind ocean current turbines. Journal of Ocean Engineering and Marine Energy. Springer International Publishing AG, 2017. pp. 113-123 (Journal of Ocean Engineering and Marine Energy).
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Pyakurel, P, Tian, W, VanZwieten, JH & Dhanak, M 2017, Characterization of the mean flow field in the far wake region behind ocean current turbines. in Journal of Ocean Engineering and Marine Energy. Journal of Ocean Engineering and Marine Energy, vol. 3, Springer International Publishing AG, pp. 113-123. https://doi.org/10.1007/s40722-017-0075-9

Characterization of the mean flow field in the far wake region behind ocean current turbines. / Pyakurel, Parakram; Tian, Wenlong; VanZwieten, James H.; Dhanak, Manhar.

Journal of Ocean Engineering and Marine Energy. Springer International Publishing AG, 2017. p. 113-123 (Journal of Ocean Engineering and Marine Energy; Vol. 3).

Research output: Chapter in Book/Report/Published conference proceedingChapter

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N2 - Methoxypolyethylene glycol (mPEG) covalently bound to the surface of human red blood cells (hRBCs) has been shown to decrease immunological recognition of hRBC surface antigens (Bradley et al., 2002). However, there is an increasing shortage of hRBC donations, thus making hRBCs scarce and expensive (Davey, 2004; Riess, 2001). The goal of this study is to similarly PEGylate the surface of bovine RBCs (bRBCs) with the aim of reducing the demand on human blood donations needed for blood transfusions. This study investigates the feasibility of modifying the surface of bRBCs with the succinimidyl ester of methoxypolyethylene glycol propionic acid (SPA-mPEG) for use as a potential blood substitute. The oxygen binding affinity of PEGylated bRBCs was moderately increased with increasing initial SPA-mPEG concentrations up to 4 mM when reacted with bRBCs at a hematocrit of 12%. Oxygen transport simulations verified that SPA-mPEG conjugated bRBCs could still transport oxygen to pancreatic islet tissues even under extreme conditions. PEGylated bRBCs reconstituted to a hematocrit of 40% exhibited viscosities on the order of 3 cp, similar to hRBCs at the same hematocrit. Taken together, the results of this study demonstrate the success of PEGylating bRBCs to yield modified cells with oxygen binding, transport and flow properties similar to that of hRBCs

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M3 - Chapter

SN - 0021-8782 (Print)\r0021-8782 (Linking)

T3 - Journal of Ocean Engineering and Marine Energy

SP - 113

EP - 123

BT - Journal of Ocean Engineering and Marine Energy

PB - Springer International Publishing AG

ER -

Pyakurel P, Tian W, VanZwieten JH, Dhanak M. Characterization of the mean flow field in the far wake region behind ocean current turbines. In Journal of Ocean Engineering and Marine Energy. Springer International Publishing AG. 2017. p. 113-123. (Journal of Ocean Engineering and Marine Energy). https://doi.org/10.1007/s40722-017-0075-9