ARL5b inhibits human rhinovirus 16 propagation and impairs macrophage-mediated bacterial clearance

Suzanne Faure-Dupuy, Jamil Jubrail, Manon Depierre, Kshanti Africano-Gomez, Lisa Öberg, Elisabeth Israelsson, Kristofer Thörn, Cédric Delevoye, Flavia Castellano, Floriane Herit, Thomas Guilbert, David G Russell, Gaell Mayer, Danen M Cunoosamy, Nisha Kurian, Florence Niedergang

Research output: Contribution to journalArticlepeer-review


Human rhinovirus is the most frequently isolated virus during severe exacerbations of chronic respiratory diseases, like chronic obstructive pulmonary disease. In this disease, alveolar macrophages display significantly diminished phagocytic functions that could be associated with bacterial superinfections. However, how human rhinovirus affects the functions of macrophages is largely unknown. Macrophages treated with HRV16 demonstrate deficient bacteria-killing activity, impaired phagolysosome biogenesis, and altered intracellular compartments. Using RNA sequencing, we identify the small GTPase ARL5b to be upregulated by the virus in primary human macrophages. Importantly, depletion of ARL5b rescues bacterial clearance and localization of endosomal markers in macrophages upon HRV16 exposure. In permissive cells, depletion of ARL5b increases the secretion of HRV16 virions. Thus, we identify ARL5b as a novel regulator of intracellular trafficking dynamics and phagolysosomal biogenesis in macrophages and as a restriction factor of HRV16 in permissive cells.
Original languageEnglish
Pages (from-to)1156-1175
Number of pages20
JournalEMBO Reports
Issue number3
Early online date8 Feb 2024
Publication statusPublished - 12 Mar 2024

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