Mixed reality (MR) is a cutting-edge technology at the forefront of many new applications in the tourism and cultural heritage sector. This study aims to reshape the museum experience by creating a highly engaging and immersive museum experience for visitors combing real-time visual, audio information and computer-generated images (CGI) with museum artefacts and customer displays. This research introduces a theoretical framework that assesses the potential of MR guidance system in usefulness, ease of use, enjoyment, interactivity, touring and future applications. The evaluation introduces the MuseumEye MR application in the Egyptian Museum, Cairo using mixed method surveys and a sample of 171 participants. The results of the questionnaire highlighted the importance of the mediating the role of the tour guide in enhancing the relationship between; perceived usefulness, ease of use, multimedia, UI design, interactivity, and the intention of use. Furthermore, the results of this study revealed the potential future use of MR in museums and ensured sustainability and engagement past the traditional visitor museum experience, which heightens the economic state of museums and cultural heritage sectors.